ADENOSINE DEAMINASE AND ITS ISOENZYMES IN ASCITES WITH DIFFERENT ETIOLOGIES

Mohamed Ahmed Abdelmoaty, Ahmed Mohamed Bogdady Bogdady

Abstract


Adenosine deaminase (ADA) activity was increased in certain clinical conditions including tuberculosis and bacterial infections. In this study, the ADA isoenzymes patterns were assayed in ascites of different etiologies Ninety two Patients with ascites were selected and investigated to determine the cause of ascites. Total ADA and its isoenzymes were assayed spectophotometerically besides polyacrylamide gel electrophoretic. Results. In ascitic fluid secondary to parainfection, TB, abdominal cancer and liver cirrhosis, total ADA were 34.5± 11.1, 87.6± 23.6, 32.7± 10.1 and 28.5± 7.3 U/l respectivly, ADA1m were 24.5± 11.1, 4.3± 1.9, 2.8± 2.2 and 10.1± 3.3 U/l respectivly, ADA1c were 7.8± 3, 16.5± 6.2, 4.7± 1.3 and 10.1± 3.3 U/l respectivly but, ADA2 were 2.2± 3.9, 65.9±  33.5, 26.2± 6.2 and 3.3± 9.7 U/l respectivly. Conclusion Total ADA above 41 U/l and ADA2 above 32 U/l in ascetic fluid have high sensitivity value in TB peritonitis. Ascitic fluid ADA1m/ADA more than 50% has high specificty value in parainfective peritonitis. Total ascitic ADA less than 41 U/L and ADA2/ADA ratio more than 50% have high specificity in abdominal cancer.

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DOI: http://dx.doi.org/10.17628/ecb.2015.4.539-542

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