Aqueous and Alcohol Extraction Of Plant (Glycyrrhiza glabra) And Activity Study Against Bacteria and Cancer (Cell Line RD)

Omar Hamad Shehab Al-Obaidi

Abstract


Isolation had been done for active substances from Glycyrrhiza glabra plant such as Saponins , volatile oils and Tannins. The percentage in form was (41.1%), ( 2.5%) and (13.2%) respectively. As well as the appointment of some mineral elements in the Glycyrrhiza glabra seeds like sodium,  calcium and potassium and the concentration in the sample was (203)ppm, (176) ppm, (181) ppm, respectively, measured by using Flame Spectrometer. Also a study of anti-bacterial activity of extracts from Glycyrrhiza glabra using two types of pathogenic bacteria Escherichia Coli and aurous Staphylococcus showed the ability of inhibition for all different extracts by vary inhibition diameters for different active substances, concentrations and bacteria. One type of cancer cellular line used to study the effect of Glycyrrhiza glabra extracts (after the chemical assay) were studied on the  growth of Rhabdomyo sarcomas (RD) cell line in human by using in vitro system and  compared with anticancer drug cisplatin (cis-pt)  as a positive control.  The cancer cell were treated with different concentration for each of the three treatments and cis-pt after 72 hr. exposure time. The cytotoxic activity was tested by inhibition rate as parameter. The results showed significant differences (p<0.05) for each three treatments when the inhibition rates were increased. There was strong correlation between the three treatments and the different concentrations in comparison with cisplatin.


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DOI: http://dx.doi.org/10.17628/ecb.2014.3.133-137

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